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Alomone Labs
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ProSci Incorporated
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PhosphoSolutions
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Image Search Results
Journal: Cancer Cell International
Article Title: LncRNA XIST from the bone marrow mesenchymal stem cell derived exosome promotes osteosarcoma growth and metastasis through miR-655/ACLY signal
doi: 10.1186/s12935-022-02746-0
Figure Lengend Snippet: BMSCs derived exosomal XIST regulated ACLY expression in osteosarcoma cells by binding miR-655. A Western blot analysis the effect of BMSCs derived exosomal XIST combined with miR-655 on the expression of ARPP19, TOB1 and ACLY in osteosarcoma cells (n = 3); B At animal level, western blot analysis the effect of BMSCs derived exosomal XIST combined with miR-655 on ACLY expression (n = 3); C The binding pattern of miR-655 and the ACLY 3'-UTR and the corresponding mutation information; D The binding of miR-655 and the ACLY 3'-UTR and their possible sites analyzed by dual luciferase reporter gene assay (n = 3). ** represents p < 0.01
Article Snippet: The PVDF membrane was incubated with primary antibodies diluted with blocking solution overnight at 4°C respectively (GAPDH, Abcam, ab8245; Lamin B1, Abcam, ab16048; β-catenin, Abcam, ab32572; CD9, Abcam, ab263019; CD63, Abcam, ab134045; CD81, Abcam, ab79559; calnexin, CST, 2679;
Techniques: Derivative Assay, Expressing, Binding Assay, Western Blot, Mutagenesis, Luciferase, Reporter Gene Assay